Effect of haemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems.

OBJECTIVES The aim was to determine the effect of blood contamination and haemostatic agent application on the shear bond strength (SBS) of different adhesives to dentin. MATERIALS AND METHODS Seventy-two extracted human molars were used in this study. Teeth were mounted acrylic in moulds. Mesial and distal surfaces were removed to obtain flat dentin surfaces (n=144) and grinded with a 600 grit sandpaper. The samples were randomly divided into three main groups (N=48) based on the adhesive system and application procedures. GROUP 1: No contamination (control), GROUP 2: Blood contamination, GROUP 3: Blood Contamination+Haemostatic agent application. Each group was further divided into four subgroups: SUBGROUP I: Single Bond 2 (etch-and-rinse) Subgroup II: Clearfil SE Bond (two-step self-etch) Subgroup III: Single Bond Universal (multimode, etch-and-rinse) subgroup IV: Single Bond Universal (multimode, all-in-one self-etch) (n=12). Resin cylinders (Filtek Z550) were bonded to the dentin surfaces according to the manufacturers' instructions. A shear load was applied to the specimens using universal testing machine at a cross-head speed of 0.5 mm/min until failure. Data were analyzed statistically (p<0.05). RESULTS Statistically significant differences were observed between no contamination (control) and blood + haemostatic agent contamination groups for both Single Bond 2 (etch-and-rinse) and Single Bond Universal (multimode, all-in-one self-etch) (p<0.05). When the adhesive systems were compared to each other, no statistically significant differences were found in all contamination groups and control group for the mean SBS values (p>0.05). CONCLUSION When blood contamination is inevitable two step self- etching adhesive systems may be the choice of adhesive system in terms of shear bond strength.